FIV/FeLV PCR Panel

FIV/FeLV PCR Panel

Comprehensive Molecular Testing for Feline Retroviruses

 

 

Accurate PCR detection of Feline Immunodeficiency Virus (FIV) and Feline Leukemia Virus (FeLV) from whole blood.

Designed to identify active infection and support early diagnosis, clinical management, and screening of at-risk cats.

Unlike screening tests that detect antibodies or viral antigen, PCR identifies viral nucleic acid, providing direct evidence of infection. Molecular testing can be especially valuable when screening results are inconclusive, when early infection is suspected, or when additional confirmation is clinically indicated.

 

 

Why Test for FIV and FeLV?

FIV and FeLV are among the most significant infectious diseases affecting domestic cats worldwide. Both viruses weaken the immune system and predispose cats to chronic infections, hematologic disorders, inflammatory disease, and certain types of cancer.

 

Early identification allows veterinarians to:

 

  • Develop appropriate treatment plans
  • Monitor infected cats more effectively
  • Reduce transmission to other cats
  • Make informed breeding and adoption decisions
  • Improve long-term patient management

 

 

What Does This Panel Detect?

 

Feline Leukemia Virus (FeLV)

FeLV is a contagious retrovirus transmitted primarily through saliva, close contact, grooming, bite wounds, shared food bowls, and from queen to kittens.

Clinical manifestations may include:

  • Immunosuppression
  • Anemia
  • Persistent fever
  • Weight loss
  • Chronic infections
  • Lymphoma and leukemia
  • Bone marrow disorders
  • Poor wound healing

 

 

Feline Immunodeficiency Virus (FIV)

FIV primarily spreads through bite wounds and is more commonly diagnosed in outdoor or free-roaming adult cats.

Many infected cats remain clinically normal for years before developing signs associated with immune dysfunction.

 

Possible clinical signs include:

 

  • Chronic oral inflammation
  • Gingivitis and stomatitis
  • Recurrent skin infections
  • Upper respiratory disease
  • Persistent fever
  • Enlarged lymph nodes
  • Weight loss
  • Poor response to treatment

 

 

Why Choose PCR?

Real-time PCR detects viral genetic material rather than relying on the host immune response.

 

Advantages include:

 

✓ Direct detection of viral DNA/RNA

✓ High analytical sensitivity

✓ High analytical specificity

✓ Supports confirmation of screening test results

✓ Useful when clinical signs and rapid test results do not agree

✓ Valuable in diagnostically challenging cases

 

 

Clinical Applications

 

The FIV/FeLV PCR Panel is recommended for:

 

  • Cats with chronic or recurrent illness
  • Cats presenting with unexplained fever
  • Persistent anemia
  • Leukopenia or other hematologic abnormalities
  • Weight loss of unknown origin
  • Chronic gingivitis or stomatitis
  • Enlarged lymph nodes
  • Newly adopted cats
  • Shelter intake screening
  • Multi-cat households
  • Breeding catteries
  • Pre-breeding evaluations
  • Blood donor screening
  • Confirmation of positive screening tests
  • Investigation of inconclusive SNAP® or ELISA results

 

 

Understanding PCR and Routine Screening Tests

PCR and routine screening assays evaluate different aspects of infection.

 

Routine screening tests typically detect:

  • FeLV p27 antigen
  • FIV antibodies

 

PCR detects:

  • Viral nucleic acid

 

Because these methods measure different biological targets, PCR is often used as a complementary diagnostic tool when confirmation or further investigation is required.

 

 

 

Benefits of Molecular Testing

 

The HealthGene FIV/FeLV PCR Panel provides veterinarians with:

  • Rapid molecular diagnosis
  • Reliable confirmation of infection
  • Improved diagnostic confidence
  • Earlier clinical decision-making
  • Comprehensive support for feline infectious disease management

 

 

 

 

Frequently Asked Questions

 

Can PCR replace a SNAP® FIV/FeLV test?

PCR should be considered a complementary diagnostic tool. Screening assays remain valuable for routine wellness testing, while PCR provides additional molecular information that may assist in confirming or further investigating infection.

 

 

When is PCR most useful?

 

PCR may be considered when:

  • Clinical signs do not match screening test results
  • Confirmation of infection is desired
  • Persistent or unexplained illness is present
  • High-risk cats require additional evaluation

 

 

Which cats should be tested?

 

Testing is recommended for:

  • Sick cats
  • Newly adopted cats
  • Cats entering multi-cat households
  • Outdoor cats
  • Shelter and rescue cats
  • Cats intended for breeding
  • Blood donor cats

 

 

Related Tests

 

Veterinarians investigating infectious disease may also consider:

  • Complete Blood Count (CBC)
  • Chemistry Profile
  • Feline Respiratory PCR Panel
  • Feline Gastrointestinal PCR Panel
  • Feline Vector-Borne Disease Panel
  • Urinalysis